A general practitioner (GP) who cares for elderly patients discharged from hospital to a home or nursing home faces the challenges of healthcare-associated infections (HAIs). An antimicrobial dry fog disinfection (DFD is a new modality of multimodal infection control strategy.To assess the effectiveness of antimicrobial DFD disinfection in the open healthcare microenvironment of a long-term care department consists of 10 (2-3 bed) rooms, average of 252 patients hospitalized annually average of 21 days. Most of patients are discharged to home care and to nursing home.We analysed the microbiological contamination of surfaces and air in patient rooms.I.We analysed the microbiological contamination of surfaces. Before the DFD application, we detected: Staphylococcus epidermidis, a potential cause of HAIs, Enterobacteriaceae and Pseudomonas aeruginosa, clinically serious pathogens (urinary, respiratory tract and wound infections), Bacillus spp. and Micrococcus spp., a common part of the environment, but indicate general contamination. In one sample, Penicillium spp. was also present, indicates the presence of Mold. After the application of DFD, all tested surfaces were negative for pathogens such as Staphylococcus, aureus, Pseudomonas aeruginosa, Enterobacteriaceae or yeast. Only occasionally were detected Bacillus spp. and Micrococcus spp., which are considered non-pathogenic, environmental bacteria, naturally present in the environment. II.We analysed the microbiological contamination of air. Before disinfection, the total number of microorganisms reached 304 – 393 CFU/m3, which represents high environmental contamination. Molds were captured in the cut (118 – 142 Colony Forming Units(CFU)/ m3). Yeasts and Staphylococcus spp. were not detected before disinfection. After DFD the total number of microorganisms dropped to 73 – 102 CFU/m3.  Molds were reduced to 53 – 73 CFU/ m3. Yeasts and Staphylococcus spp. were not detected even after disinfection. DFD reduced the microbial load of the air by 70-80%.The practice requires further studies in the future so that the effectiveness of DFD can be assessed more comprehensivelyThe use of DFD has the potential to significantly reduce the microbial burden as part of a comprehensive HAI prevention strategy in department predominantly caring of frail patients and then facilitate the work of GPs after discharge.